Two turkeys, named Peanut Butter and Jelly, were pardoned during the traditional ceremony in the Rose garden that was attended by some 100 guests.
During his speech, Biden noted that while many people this year will be able to gather at the table with their families and friends after a hard pandemic year and many with empty seats will be kept in prayers.
"With the powers vested in me as the president of the United States, I pardon you," Biden said.
Biden also revealed that after receiving their presidential pardon, Peanut Butter and Jelly will be headed to Perdue University in West Lafayette, Indiana.
The tradition of sending turkeys to US presidents for the Thanksgiving holidays dates back to the 19th century. The first official Thanksgiving turkey pardoning was performed by US President George H. W. Bush in 1989.
"Eventually, Peanut Butter and Jelly were selected based on their temperament, appearance, and, I suspect, vaccination status," Biden joked.