"We have purposefully designed these sanctions to maximise a long-term impact on Russia and to minimise the impact on the United States and our allies", he said.
He announced four more Russian banks that hold more than $1 trillion in assets would be sanctioned and their assets in the United States frozen, and that Russian "elites" and their families would be sanctioned as well. He said sanctioning Russian President Vladimir Putin is also "on the table".
"These are people who personally gained from the Kremlin's policies and they should share in the pain", Biden said of those sanctioned. "We will keep up this drumbeat of those designations against corrupt billionaires in the days ahead".
The Treasury additionally sanctioned 24 Belarusian individuals and entities, including Belinvestbank and Bank Dabrabyt, as well as close affiliates of President Alexander Lukashenko.
Early on Thursday morning, Putin announced the beginning of a military operation in Ukraine to end the constant attacks on the Donetsk and Lugansk People's Republics, which his government recognised earlier this week. However, Biden has claimed that the operation is the "full-scale invasion" that his government has predicted was imminent for several months.
Since 2017, US military strategy documents have described Russia as a "malign actor" alongside China, against both of which the US must engage in "great power competition", abandoning its two-decade War on Terror.