US President Donald Trump warned Tehran on Sunday of consequences "the likes of which few throughout history have ever suffered," if the country threatens the United States.
The Iranian president said earlier that a "war with Iran is the mother of all wars," but did not rule out peace between the two countries.
"Today, speaking with US has no meaning except surrender and end of people's achievements. Mr. Trump! We are the people of dignity and guarantor of security of the waterway of the region throughout the history. Don't play with the lion's tail; you will regret it," Rouhani said at a meeting with Iranian diplomats as quoted by the presidential office.
US Secretary of State Michael Pompeo delivered a speech Sunday entitled "Supporting Iranian Voices" at the Ronald Reagan Presidential Library and Center for Public Affairs, in which he compared the Iranian government to the mafia.
"Iran is run by something that resembles the mafia more than a government," Pompeo said
In addition, the US president decided to reinstate wide-ranging sanctions on Iran, previously lifted under the agreement in exchange for Tehran maintaining the peaceful nature of its nuclear program. Washington's unilateral move has been opposed by other parties to the nuclear deal.