"Tropical Storm #Eta has made landfall on Lower Matecumbe Key Florida at 11pm EST [04:00 GMT on Monday]", NHC said on Twitter.
The maximum sustained winds were 65 miles per hour and the storm is currently moving northwest at 13.6 miles per hour.
On Sunday, Eta reached Cuba and hit southern regions of the central part of the country's coast.
Tropical storm Eta, a Category 4 hurricane at its peak, caused heavy rainfall in parts of Central America.
Floods affected Nicaragua, Honduras, El Salvador, Guatemala, Panama, and Costa Rica. According to preliminary data from the authorities, more than 170 people died in these countries as a result of the disaster.