Eight women wearing all black used painted hammers with words such as LIFE, LOVE and CARE on them to break glass at the "World’s worst bank" and before doing so, they stuck stickers to the windows that read "In case of climate emergency, break glass", the environmental group said in a statement.
After that, protesters sat on the road in a circle to wait for the police to arrest them.
During the so-called "Impossible Rebellion" that started on 23 August, climate activists have repeatedly targeted the City of London, heart of the UK's financial industry, accusing banks of contributing to global warming by investing in and funding fossil fuel companies.
Last week, protesters splattered red paint over the facade of the Standard Chartered Bank and London's Guildhall, the building housing the City of London Corporation, and created nonviolent chaos in several parts of the British capital.
On Tuesday, the Metropolitan Police said there had been a total of 367 arrests in London during the first week of climate protests.