"I am clear that what we have seen shows that there is no other conclusion but the Russian state is culpable for what happened on the streets of Salisbury," May said, rejecting the denial by Russian President Vladimir Putin.
The UK prime minister's claim comes the day after Putin called the allegations of Russia's involvement in the poisoning "nonsense," as Russia does not even have chemical weapons, because it destroyed all of it, unlike a number of its international partners.
READ MORE: PM May's Anti-Russia Stance Largely for Own Political Gain, Not UK Security
Earlier in the day, presidential spokesman Dmitry Peskov noted that a phone conversation between the Russian president and the UK prime minister had not been planned yet.
Russia denied all accusations and expressed readiness to collaborate on the investigation. On Saturday, Russia announced retaliatory measures against the United Kingdom, declaring 23 staff of the UK Embassy in Moscow personae non gratae in response to London's move.
READ MORE: UK Police See No Connection Between Skripal and Glushkov Cases
Skripal and his daughter Yulia were found unconscious earlier this month on a bench at a shopping center in Salisbury. Both of them remain in critical condition and are being treated for exposure to an А-234 chemical substance.
Previously, former Russian intelligence agent Skripal had been accused of espionage on behalf of London. He fled the country and was later granted asylum in the United Kingdom as a part of a US-Russia spy exchange.
