"Met Italy's Deputy PM Di Maio this afternoon to discuss US-Italian cooperation on the crisis in Venezuela", Bolton said via Twitter.
Italy is one of a few countries in the European Union that has declined to recognize opposition leader Juan Guaido as the interim president of Venezuela.
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Italy is currently participating in a ministerial-level meeting of the International Contact Group on Venezuela that is being held in the Ecuadorian capital of Quito.
In January, Guaido, backed by the United States, declared himself the interim president of Venezuela after disputing President Nicolas Maduro’s re-election last May. Washington immediately endorsed Guaido, called on Maduro to step down and seized billions of dollars’ worth of Venezuela’s oil assets.
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Maduro has accused the United States of trying to orchestrate a coup in order to install Guaido as a puppet leader beholden to Washington. Russia, China, Cuba, Bolivia, Turkey and a number of other countries have voiced their support for constitutionally elected Maduro as the only legitimate president of Venezuela.