"$9.5 billion for equipment for Ukraine and replenishment of [US Department of Defense] stocks; and $3.6 billion for continued military, intelligence, and other defense support," the document said.
Biden is also asking for $7.3 billion for economic, humanitarian, and security assistance to Ukraine.
The proposal for additional Ukraine aid totals some $24 billion, including some $2.3 billion intended as leverage to gain more aid from other donors via the World Bank.
However, combined with an additional $2.65 billion in funding for border security, $12 billion in disaster relief efforts, and $416 million in combatting the fentanyl crisis, among other domestic matters, the congressional request totals upwards of $40 billion in supplemental aid.
The Thursday filing marks the first such request by the Biden White House since Republicans claimed control of the House of Representatives at the start of the year. While previous requests had been largely met with Congress' backing, GOP members have grown increasingly hesitant toward continued aid.
In fact, House Speaker Kevin McCarthy vowed in early June that any requests for supplemental Ukraine aid would not be taken up in the lower chamber - regardless of bipartisan efforts taken up in the Senate.
At the time, McCarthy explained that any additional funds would need to be cleared as part of an annual appropriations process, underscoring finances would have to be shifted elsewhere from the Pentagon's funds.
“I think what we really need to do, we need to get the efficiencies in the Pentagon,” the House speaker told US media in June when referring to the Pentagon's budget. “Think about it, $886 billion. You don’t think there’s waste? … I consider myself a hawk, but I don’t want to waste money. So I think we’ve got to find efficiencies.”
Shortly after the Thursday request was issued, US Rep. Paul Gosar (R-AZ) told Sputnik that US President Joe Biden must focus on supporting efforts to improve security within the United States, rather than on spending more funding on a proxy war in Ukraine.
"Rather than spending a single penny more fighting a proxy war in Ukraine and killing more people, a more worthwhile effort would be if Biden would put America first by allocating resources in our country to secure the southern border," Gosar said, touching on the US' continued fight to combat illegal immigration.
He added that resources could also be better spent on "funding law enforcement efforts to combat the violent crime and drugs destroying cities across America, or aiding our homeless population, including countless veterans, who are sleeping on sidewalks."
The hefty request also comes as the US public has grown increasingly cold toward such military aid.
A recent poll determined that 55% of surveyed Americans opposed Congress approving Ukraine aid, with only 45% disagreeing. Earlier Wednesday, the White House attempted to shoot down the sentiment, telling reporters that continued efforts were paramount to the "national security of the American people."
US National Security Council Coordinator for Strategic Communications John Kirby touched on the polling on Thursday and opted to reiterate the White House's stance that the American people understand why Biden requested billions more in aid for Ukraine.
"The polls notwithstanding, I think the American people understand what is at stake here," Kirby said.
To date, the US has provided Ukraine with more than $100 billion in aid since the start of the conflict, with the majority of the funds being designated specifically for military equipment.