DeSantis said that he believed Trump should not have run for reelection even before being indicted given that he is a "deal-breaker" for many voters.
"I think the chance of getting elected president after being convicted of a felony is as close to zero as you can get," DeSantis said in an interview with US media.
However, more than half of Republican voters say that the criminal charges against Trump are not a serious cause for concern, according to a poll released on Wednesday.
Trump remains the frontrunner to win the 2024 Republican presidential nomination with 52% support, a 34-point advantage over DeSantis, the poll said.