"We accept all the conditions, ultimatums and arm-twisting that the Our Ukraine-People's Self-Defense Bloc has offered," Tymoshenko said.
The pro-Western alliance fell apart in September due to infighting and differences regarding policy toward Russia in the wake of its armed conflict with Georgia last month.
The pro-presidential bloc said the new coalition could be formed only if the results of the vote backed by the Yulia Tymoshenko Bloc last month to reduce the president's powers were annulled and a declaration condemning Russia's August operation in South Ossetia was issued.
The factions formerly within the ruling coalition, which also included the Supreme Rada's smallest group, led by former parliamentary speaker Volodymyr Lytvyn, have been talking about forming a new alliance of pro-Western political forces since last week.
However, talks have made no headway, raising the prospect of early elections. Former Soviet Ukraine has been plagued by political instability since 2004. The previous parliamentary election took place last September.
Tymoshenko also rejected Wednesday claims by Vyacheslav Kyrylenko, who heads the pro-presidential Our Ukraine-People's Self-Defense Bloc, that the premier was trying to oust him from the post.
"I want to say first that I'm not seeking this," she said. "This is an internal affair of the Our Ukraine-People's Self-Defense Bloc and only they can decide on who is their leader."