The EU’s black list features 149 individuals and 37 legal entities.
The current sanctions imposed on individuals and companies for their alleged participation in the Ukrainian crisis were to expire on September 15.
The latest measure came into force Monday after publication in the official journal of the European Union.
Russia's relations with the West deteriorated significantly in 2014 over the crisis in Ukraine.
The United States and the European Union accused Russia of interfering in Ukraine's internal affairs, imposing several rounds of sanctions on Moscow.
Russia has repeatedly refuted the allegations and retaliated by imposing a food import ban on several EU countries.
