Sanders supporters have been frustrated over the release of DNC emails by Wikileaks that show the supposedly neutral organization had a bias toward Clinton.
Earlier on Tuesday, Clinton was officially nominated as the party's presidential nominee, making her the first female candidate of a major US political party.
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— Cassandra Fairbanks (@CassandraRules) July 26, 2016
In an attempt at unity, Sanders had urged his supporters not to leave the arena. A number walked off the floor following Clinton's nomination, however.
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Nearly all seats in the Maine, Kansas, Alaska, and Oklahoma sections were empty.
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Protests also occurred on the first day of the convention, with speakers interrupted by boos and chants of "Lock Her Up." Many voiced their opposition to the TPP trade deal.