"We can say that the [economic] blockade has increased. This contradicts the package of measures signed in Minsk," Pushilin told RIA Novosti.
He added that the economic blockade in the region is causing grave concerns, as the "shops are emptying very quickly."
Ukraine's southeastern region of Donbas is currently facing a humanitarian crisis as a result of a military operation launched by Kiev authorities in April 2014.
The authorities of the self-proclaimed republics of Donetsk and Luhansk have repeatedly urged Kiev to remove the economic restrictions.
The warring sides held several rounds of negotiations in a bid to find a peaceful solution to the armed conflict in Donbas.
The most recent talks on Ukraine reconciliation were held in the Belarusian capital Minsk on February 12. The leaders of Germany, France, Ukraine and Russia brokered a peace deal between Kiev and Donbas independence supporters aimed at de-escalation of the east Ukraine crisis.
The measures hammered out in Minsk include a ceasefire, which became effective on February 15, heavy artillery withdrawal from the line of contact, all-for-all prisoner swap and some constitutional changes granting Donbas more autonomy.