"Of course, the actions that were taken this week were an unfriendly step. They really harmed the prospects for the restoration of our relations," Peskov told Russian journalist Pavel Zarubin.
At the same time, Washington's new sanctions are not a reason to abandon aspirations to restore relations between the two countries.
"Our interests involve creation of good relations with all countries, including the US," Peskov added.
On Wednesday, the United States unveiled a new package of sanctions against Russia, which included measures against oil giants Rosneft, Lukoil and their subsidiaries.